Biotech Core, Inc. - Links - Biotech, Core, Biotechcore, Biotech Core, Sequencing, DNA Sequencing, Mutagenesi, Super RACE Full-Length Gene Cloning, Gene Synthesis


	Phone: 1-800-463-2888
	DNA SEQUENCING SERVICES Utilizing an ABI automated DNA sequencing facility, Biotech Core delivers state-of-the-art DNA sequencing services for all major type of templates. Primer Extension Service (Call for Quotation) Sequencing reactions are performed using universal primers (M13 forward, M13 reverse, T7, T3, or SP6) or custom primers. DNA templates can be plasmid, DNA fragment, or PCR products. Up to 700 bases of information are normally provided for each sequence run. Normal turnaround time is 24 hours. Sequencing data is provided via e-mail. Single-Strand DNA Sequencing (Call for Quotation) Overlapping sequencing is performed on one strand of DNA. The resulting sequences will be assembled and edited. All required sequencing primers are designed and synthesized at no additional charge. The final analyzed DNA sequence typically contains <2% ambiguities. Guaranteed Double-Strand DNA Sequencing (Call for Quotation) Complete overlapping sequencing is performed on both DNA strands. All ambiguities are resolved by redundant sequencing. The sequences will be assembled and edited. All required primers are designed and synthesized at no additional charge. The final DNA sequence is publication guaranteed. Sample Requirement Clean DNA is essential for high quality DNA seqeuncing. Qiagen and other DNA preparation columns are recommended. Plasmid DNA quantity: 1 micrograme/reaction. DNA concentration should be 0.1-1.0 micrograme/microliter in H2O or tris buffer. PCR products: it is essential to clean up the PCR primers. PCR spin columns from Qiagen and other companies are recommended. A good single band PCR product with an amount of 0.05-1 microgrames (depending on the size of the PCR product) normally generates high quality sequencing data. A photograph developed on agarose gel (normally taken immediately after PCR reactions), indicating the size and quantity of each PCR product, must be submitted with the samples. Primer: customer primers should be 17-27mer in length, have little or no secondary structure, 35-70% GC. The primer should be submitted in 10 microliter with concentration of either 50ng/microliter, 5 micromolar, or 5 pmole/microliter in H2O or tris. Biotech Core provides universal primers (M13F, M13R, T3, T7, SP6) at no charge. OTHER MOLECULAR BIOLOGY SERVICES Mutagenesis Depending on the client's request, we can design and implement all the strategic steps necessary for the introduction of single or multiple bases mutation, deletion, and addition into genes. At the end of this process, the mutated region of each mutant (which was PCRed and swabbed in the original plasmid during the mutation) will be confirmed by our guaranteed double-strand sequencing. The finished package delivered to the client will include: the mutated plasmid DNA, bacteria clone and confirmed sequence data. Customers should provide a minimum of 2 micrograme plasmid DNA and the DNA sequence in electronic format. Call for quotation
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